Perforin Antibody (PE)

SKU: orb222762

Description

Mouse monoclonal antibody against Perforin conjugated to PE

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Bovine, Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone No.: dG9
• Immunogen: purified granules from human YT lymphoma cell line
• Target: Perforin
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRF1, P1, PFP, HPLH2

Expression profiling on peripheral blood subsets using Anti-human Perforin PE antibody (clone dG9). Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set. HCDM CDMaps standardized procedures were used for leukocyte isolation. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, EXCELLYSE XPerm buffer set was used for leukocytes permeabilization according to the instruction procedure. Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10^6 cells).

Anti-human Perforin PE antibody (clone dG9) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm solution. Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10^6 cells).

Quick Database Links

Gene Symbol

Perforin

UniProt

P14222

NCBI Gene ID

5551