Cart summary

You have no items in your shopping cart.

Anti-PDK4 Antibody

Catalog Number: orb340861

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340861
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PDK4
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide of human PDK4
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:10-1:100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPDK4
Entrez27273, 5166
UniProt IDO70571, Q16654
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesPDHK4; [Pyruvate dehydrogenase (acetyl-transferrin
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PDK4 Antibody

Western blot analysis of PDK4 expression in THP1 (A), Hela (B), mouse kidney (C), mouse heart (D) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)

Anti-PDK4 Antibody

Immunohistochemical analysis of PDK4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PDK4 Antibody

Immunofluorescent analysis of PDK4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Anti-PDK4 Antibody [orb1819497]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-PDK4 Antibody [orb1473762]

    WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • Anti-PDK4 Antibody [orb334505]

    WB

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • PDK4 Antibody [orb680177]

    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Anti-PDK4 Antibody [orb2585148]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg