Anti-PD-1 [1G4]

SKU: orb1906341

Description

Anti-PD-1 [1G4]

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC
• Reactivity: Human, Primate

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1G4
• Immunogen: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.
• Target: PD-1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD279; PD1; PD 1; hPD-1; PDCD1; Programmed cell death protein 1; Programmed cell death 1; Protein PD-1; 41-1G4; 1G4-mHvKv

Immunofluorescence staining of Molt4 cells with anti-PD-1 antibody 1G4. Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1G4 (orb1906340) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906340, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-PD-1 antibody 1G4. Human lymph node (A) and spleen (B) tissue lysates (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 1G4 (orb1906340) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Quick Database Links

Gene Symbol

PD-1

UniProt

Q15116