PCNA Antibody

SKU: orb323186

Description

The PCNA Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary. Purification: Affinity chromatography

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:2000-1:5000, IHC-P: 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Proliferating cell nuclear antigen; PCNA; Cyclin

Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 29 kD)

Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P12004, P04961, P17918

NCBI Gene ID

25737, 5111, 18538