Anti-p38 (pT180) Antibody

• Catalog Number: orb1423097
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p38 (pT180).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: MAPK14
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), CHIP: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), E: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
• Conjugation: Unconjugated
• UniProt ID: Q16539
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Direct ELISA antibody dose-response curve using Anti-p38 (pT180) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Immunofluorescent analysis of p38 (pT180) staining in HepG2 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of p38 (pT180) expression in mouse liver (A), rat liver (B) whole cell lysates.

Western blot analysis of p38 (pT180) expression in zebrafish (A) whole cell lysates.