OPAL1 Antibody

SKU: orb222816

Description

Mouse monoclonal antibody to OPAL1

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: OPAL1-01
• Immunogen: Recombinant fragment of human OPAL1 (amino acids 152-342)
• Target: OPAL1
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WBP1L

Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.

Western blotting analysis of human OPAL1 using mouse monoclonal antibody OPAL1-01 on lysates of HEK-293T/OPAL1 transfectants, and of murine splenocytes and HEK-293T cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-OPAL1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. OPAL1 was detected at around 45-60 kDa.

Quick Database Links

Gene Symbol

OPAL1

UniProt

Q9NX94

NCBI Gene ID

54838