Notch1 Antibody

SKU: orb154385

Description

Mouse monoclonal antibody to Notch 1 which belongs to the NOTCH family transmembrane receptors that plays an important role in development and the determination of cell fate. It expressed in fetal tissues most abundant in spleen, brain stem and lung. Notch 1 has functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: mN1A
• Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1
• Target: Notch1
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AOS5, TAN1, hN1, AOVD1

Separation of Jurkat cells stained using anti-Notch1 (mN1A) purified antibody (concentration in sample 16 µg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

Notch1

UniProt

P46531

NCBI Gene ID

4851