Notch1 Antibody

• Catalog Number: orb154385
• Category: Antibodies
• Description: Mouse monoclonal antibody to Notch 1 which belongs to the NOTCH family transmembrane receptors that plays an important role in development and the determination of cell fate. It expressed in fetal tissues most abundant in spleen, brain stem and lung. Notch 1 has functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
• Target: Notch1
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1
• UniProt ID: P46531
• Tested applications: FC, ICC, IHC-Fr, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: mN1A
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AOS5, TAN1, hN1, AOVD1
• Research Area: Cancer, Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 4851

Separation of Jurkat cells stained using anti-Notch1 (mN1A) purified antibody (concentration in sample 16 µg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (intracellular staining).