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Anti-MYD88 Antibody

Catalog Number: orb214286

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214286
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MyD88
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Porcine, Primate, Rat, Sheep
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYD88. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMyD88
Entrez4615, 301059, 17874
UniProt IDQ99836, Q6Y1S1, P22366
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Myeloid differentiation primary response prot
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MYD88 Antibody

Western blot analysis of MYD88 expression in HEK293T (A), Hela (B), mouse lung (C), mouse muscle (D), mouse spleen (E), rat muscle (F), rat spleen (G) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 33; 31; 28 kD)

Anti-MYD88 Antibody

Immunohistochemical analysis of MYD88 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MYD88 Antibody

Immunofluorescent analysis of MYD88 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-MyD88 Antibody [orb215902]

    ELISA,  FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    ELISA,  FC,  IF,  IHC,  WB

    Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
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    ELISA,  IHC,  WB

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg
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    ELISA,  FC,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    FC,  ICC,  IF,  IHC,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl