anti-mScarlet

• Catalog Number: orb1463262
• Category: Antibodies
• Description: Goat polyclonal antibody to mScarlet (Scarlet fluorescent protein). mScarlet is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5 kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IEM, IF, IHC-Fr, IHC-P, WB
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant fluorescent protein produced in E. coli.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
• Conjugation: Unconjugated
• Target: In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein)
• Source: Purified recombinant fluorescent protein produced in E. coli
• Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: red fluorescent protein antibody.
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence hCEC cells lysate using mScarlet antibody

WB analysis of HEK293 transfected cell lysates at 50 µg per lane using mScarlet antibody

Immunofluorescence analysis of Drosophila larvae NMJ muscle 6/7 expressing mScarlet in neurons (DyGlutmScarlet) using mScarlet antibody

Immunofluorescence analysis of hCEC cells using mScarlet antibody