CD9 Antibody (Biotin)

SKU: orb154465

Description

Mouse monoclonal antibody conjugated to Biotin which recognizes CD9 that is amember of the tetraspanin (TM4SF) family. It expressed by a variety of hematopoietic and epithelial cells. It involved in platelet activation, cell adhesion, cell motility and tumor metastasis and aggregation and also regulates paranodal junction formation.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IP, WB
• Reactivity: Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 2-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG1
• Clone No.: EM-04
• Immunogen: Permeabilized murine bone marrow-derived mast cells (BMMC).
• Target: CD9
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mic3, Mrp-1, Btcc-1, Drap-27, Tspan-29

Flow cytometry multicolor surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC) and SYTO16.

Separation of murine myeloid cells (red-filled) from CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Quick Database Links

Gene Symbol

CD9

UniProt

P40240

NCBI Gene ID

12527