CD9 Antibody (Biotin)

SKU: orb154465

Description

Mouse monoclonal antibody conjugated to Biotin which recognizes CD9 that is amember of the tetraspanin (TM4SF) family. It expressed by a variety of hematopoietic and epithelial cells. It involved in platelet activation, cell adhesion, cell motility and tumor metastasis and aggregation and also regulates paranodal junction formation.

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: FC, ICC, IP, WB
• Reactivity: Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 2-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG1
• Clone No.: EM-04
• Immunogen: Permeabilized murine bone marrow-derived mast cells (BMMC).
• Target: CD9
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mic3, Mrp-1, Btcc-1, Drap-27, Tspan-29

Flow cytometry multicolor surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC) and SYTO16.

Separation of murine myeloid cells (red-filled) from CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Quick Database Links

Gene Symbol

CD9

UniProt

P40240

NCBI Gene ID

12527