CD154 Antibody

SKU: orb44601

Description

Hamster Monoclonal to CD154.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FA, FC, ICC, IHC, IP
• Reactivity: Mouse
• Application Notes:
  Functional application: Blocking T cell costimulation in vivo. Flow cytometry: Recommended dilution: 4 μg/ml; positive control: 6-8 hour activated mouse splenocytes.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG1
• Clone No.: MR-1
• Immunogen: Activated mouse Th1 clone D1.6
• Target: CD154
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) purified antibody (low endotoxin, concentration in sample 3 μg/ml, GAArH APC).

Flow cytometry surface staining pattern of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) purified antibody (low endotoxin, concentration in sample 3 μg/ml, GAArH APC).

Quick Database Links

Gene Symbol

CD154

UniProt

P29965

NCBI Gene ID

959