CD11b Antibody

SKU: orb44821

Description

Rat monoclonal antibody to CD11b

Research Area

Neuroscience

Images & Validation

• Tested Applications: FA, FC, IHC-Fr, IP
• Reactivity: Human, Mouse, Primate, Rabbit
• Application Notes:
  Functional application: In vitro blocking of CD11b. Flow cytometry: Recommended dilution: 1 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Clone No.: M1/70
• Immunogen: B10 mouse spleen cells enriched for T cells
• Target: CD11b
• Purification: Purified by protein-G affinity chromatography followed by size-exclusion chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD11b (M1/70) purified antibody (low endotoxin, concentration in sample 0.6 μg/ml) DAR APC.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD11b (M1/70) purified antibody (low endotoxin, concentration in sample 0.6 μg/ml) DAR APC.

Quick Database Links

Gene Symbol

CD11b

UniProt

G5E8F1

NCBI Gene ID

16409