Anti-MOG [M3-8]

SKU: orb1821648

Description

Anti-MOG [M3-8]

Images & Validation

• Tested Applications: Blocking, ELISA, IHC
• Reactivity: Rat

Key Properties

• Host: Monkey
• Clonality: Monoclonal
• Isotype: Monkey IgG1
• Clone No.: M3-8
• Immunogen: Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
• Target: MOG

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Myelin oligodendrocyte glycoprotein; rMOG

Quick Database Links

Gene Symbol

MOG

UniProt

Q63348