Mitofusin 1 MFN1 Antibody (monoclonal, 3H3)

• Catalog Number: orb623774
• Category: Antibodies
• Description: Anti-Mitofusin 1 MFN1 Antibody (monoclonal, 3H3). Tested in IF, ICC, WB applications. This antibody reacts with Human.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.
• UniProt ID: Q8IWA4
• MW: 84 kDa
• Tested applications: ICC, IF, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
• Antibody Type: Primary Antibody
• Clone Number: 3H3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Fzo homolog antibody; Hfzo1 antibody; Hfzo2 antibo
• Note: For research use only

IF analysis of MFN1 using anti-MFN1 antibody. MFN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL mouse anti-MFN1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of MFN1 using anti-MFN1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela tissue lysates, Lane 2: human HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MFN1antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFN1 at approximately 84 KD. The expected band size for MFN1 is at 84 KD.