MECP2 Mouse Monoclonal Antibody

SKU: orb654396

Description

Anti-MECP2 Antibody (monoclonal, 2G3). Tested in WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 2G3
• Immunogen: E.coli-derived human MECP2 recombinant protein (Position: K119-R453).
• Target: Methyl-CpG-binding protein 2
• Molecular Weight: 75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AUTSX3; MeCp 2 protein; MECP2; Methyl CpG binding protein 2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RTS; RTT

Western blot analysis of MECP2 using anti-MECP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MECP2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75 KD. The expected band size for MECP2 is at 75 KD.

Quick Database Links

Gene Symbol

Methyl-CpG-binding protein 2

UniProt

P51608