MBP Mouse Monoclonal Antibody

SKU: orb1474103

Description

The MBP Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human MBP. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/1000 - 1/5000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Immunogen: Recombinant fusion protein of human MBP. The exact sequence is proprietary.
• Target: MBP
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: crude ascites with 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

Western blot analysis of MBP expression in ZR751 (A) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 32 kD)

Immunofluorescent analysis of MBP staining in NCIH460 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MBP

UniProt

P02686

NCBI Gene ID

4155