Anti-MAP1B Antibody

• Catalog Number: orb667223
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAP1B
• Target: MAP1B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human MAP1B. The exact sequence is proprietary.
• UniProt ID: P46821, P14873, P15205
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Microtubule-associated protein 1B antibody, a
• Note: For research use only
• Entrez: 4131, 17755, 29456

Western blot analysis of MAP1B expression in HT1080 (A), rat testis (B) whole cell lysates. (Predicted band size: 270 kD; Observed band size: 350 kD)

Immunofluorescent analysis of MAP1B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.