LRRC32 Rabbit Polyclonal Antibody

SKU: orb623870

Description

Anti-LRRC32 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Research Area

Immunology & Inflammation, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human ELISA, 0.1-0.5μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human LRRC32 recombinant protein (Position: H20-R544).
• Target: Transforming growth factor beta activator LRRC32
• Molecular Weight: 72 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D11S833E; GARP; Garpin; LRRC32

Western blot analysis of LRRC32 using anti-LRRC32 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72 KD. The expected band size for LRRC32 is at 72 KD.

Quick Database Links

Gene Symbol

Transforming growth factor beta activator LRRC32

UniProt

Q14392