Anti-Laminin gamma 1 Antibody

• Catalog Number: orb665911
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Laminin gamma 1
• Target: LAMC1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant protein of human Laminin gamma 1. The exact sequence is proprietary.
• UniProt ID: P02468, P11047
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1000, IHC-P: 1-100-200, IF: 1-100-500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-LAMB2 antibody, anti-Laminin subunit gamma-1
• Note: For research use only
• Entrez: 3915

Western blot analysis of Laminin gamma 1 expression in LO2 (A), mouse kidney (B), rat kidney (C) whole cell lysates. (Predicted band size: 177 kD; Observed band size: 220 kD)

Immunohistochemical analysis of Laminin gamma 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.