Anti-Laminin-2 LG5 [C3_Hu10]

SKU: orb1821532

Description

Anti-Laminin-2 LG5 [C3_Hu10]

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: C3_Hu10
• Immunogen: The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.
• Target: Laminin-2 LG5
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Merosin; Laminin subunit alpha-2; LAMA2; Laminin 211; laminin G-like 5 domain; LG5; g-like 4 and 5 domain; LG-4/5; Laminin M chain; Laminin-12 subunit alpha; Laminin-2 subunit alpha; Laminin-4 subunit alpha; Merosin heavy chain; C3; humanized C3

Immunofluorescence staining of HeLa cells with anti-Laminin-2 LG5 antibody C3_Hu10. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of C3_Hu10 (orb1821531) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821531, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Laminin-2 LG5

UniProt

P24043, Q60675