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Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | This antibody is generated from mouse immunized with Kappa light chain recombinant protein. |
| Purification | This antibody is purified through a protein G column. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG1 kappa. Liquid in PBS, and 0.01% sodium azide. |
| Disclaimer | For research use only |
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Western blot analysis of Kappa light chain expression in human serum (A) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 27 kD)

Immunohistochemical analysis of Kappa light chain staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
Kappa light chain Antibody (orb1474176)
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