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IRS1 Antibody
SKU: orb215977
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively. |
| Target | Insulin receptor substrate 1 |
| Molecular Weight | 132 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Insulin receptor substrate 1; IRS-1; IRS1
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Anti-IRS1 antibody, Western blotting. All lanes: Anti IRS1 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: MM453 Whole Cell Lysate at 40 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Predicted bind size: 132 KD. Observed bind size: 132 KD.
Anti-IRS1 antibody, Western blotting. All lanes: Anti IRS1 at 0.5 ug/ml. WB: Recombinant Human IRS1 Protein 0.5 ng. Predicted bind size: 39 KD. Observed bind size: 39 KD.
Anti-IRS1 antibody, IHC(P): Human Intestinal Cancer Tissue.
Anti-IRS1 antibody, IHC(P): Human Lung Cancer Tissue.
Anti-IRS1 antibody, IHC(P): Mouse Intestine Tissue.
Anti-IRS1 antibody, IHC(P): Rat Intestine Tissue.
Anti-IRS1 antibody, IHC(P): Rat Skeletal Muscle Tissue.
Quick Database Links
Gene Symbol
Insulin receptor substrate 1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
IRS1 Antibody (orb215977)
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