IRS1 Rabbit Polyclonal Antibody

SKU: orb215977

Description

IRS1 Rabbit Polyclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively.
• Target: Insulin receptor substrate 1
• Molecular Weight: 132 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Insulin receptor substrate 1; IRS-1; IRS1

Anti-IRS1 antibody, Western blotting. All lanes: Anti IRS1 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: MM453 Whole Cell Lysate at 40 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Predicted bind size: 132 KD. Observed bind size: 132 KD.

Anti-IRS1 antibody, Western blotting. All lanes: Anti IRS1 at 0.5 ug/ml. WB: Recombinant Human IRS1 Protein 0.5 ng. Predicted bind size: 39 KD. Observed bind size: 39 KD.

Anti-IRS1 antibody, IHC(P): Human Intestinal Cancer Tissue.

Anti-IRS1 antibody, IHC(P): Human Lung Cancer Tissue.

Anti-IRS1 antibody, IHC(P): Mouse Intestine Tissue.

Anti-IRS1 antibody, IHC(P): Rat Intestine Tissue.

Anti-IRS1 antibody, IHC(P): Rat Skeletal Muscle Tissue.

Quick Database Links

Gene Symbol

Insulin receptor substrate 1

UniProt

P35568