IREB2 Rabbit Polyclonal Antibody

SKU: orb1289734

Description

The IREB2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human IREB2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to human IREB2. The exact sequence is proprietary.
• Target: IREB2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2

Western blot analysis of IREB2 expression in HEK293T (A), mouse kidney (B), rat liver (C) whole cell lysates. (Predicted band size: 37; 105 kD; Observed band size: 105 kD)

Immunofluorescent analysis of IREB2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

IREB2

UniProt

Q811J3, P48200, Q62751

NCBI Gene ID

3658, 64602, 64831