Anti-Integrin beta-7 [FIB504]

• Catalog Number: orb613882
• Category: Antibodies
• Description: Rat monoclonal antibody to Integrin beta7
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: FIB504
• Tested applications: Blocking, FC, IF
• Reactivity: Human, Mouse
• Isotype: IgG2a
• Immunogen: FIB504 was prepared by immunizing rats with TK1 cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Integrin beta-7
• UniProt ID: P26011
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FIB-27; FIB 27; Fib-27; Fib27; Fib 27; fib27; fib-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 (orb613881) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613881, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.