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Anti-IL-21/Il21 Antibody
Catalog Number: orb692190
| Catalog Number | orb692190 |
|---|---|
| Category | Antibodies |
| Description | Anti-IL-21/Il21 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E.coli-derived mouse IL-21/Il21 recombinant protein (Position: H18-S146). |
| UniProt ID | Q9ES17 |
| MW | 65-80 kDa |
| Tested applications | ELISA, IHC |
| Application notes | Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500μg/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Beta-2-microglobulin; B2m |
| Note | For research use only |
IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody. IL-21/Il21 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-21/Il21 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody. IL-21/Il21 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-21/Il21 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
