Anti-IFN gamma [D9D10]

• Catalog Number: orb613795
• Category: Antibodies
• Description: Mouse monoclonal antibody to IFN gamma
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: D9D10
• Tested applications: Blocking, ELISA, FC, WB
• Reactivity: Human, Monkey
• Isotype: IgG1
• Immunogen: FPLC-purified natural human IFN-y. Refer to the original article [PMID: 3114127]
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IFN gamma
• UniProt ID: P01579
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interferon gamma; Immune interferon ; IFN-gamma; I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-IFN gamma antibody D9D10 Human thymus lysate (35 µg protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of D9D10 (orb613796) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for IFN gamma is 19.3kDa, though the form shown here is likely glycosylated. orb613796 successfully detected IFN gamma in human thymus samples.