anti-Human IgG, HRP

• Catalog Number: orb1463301
• Category: Antibodies
• Description: Goat polyclonal antibody to human IgG. IgG is a monomeric immunoglobulin, with two heavy chains and two light chains. IgG can be found in blood and is the most abundant immunoglobulin, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses (IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%)) that can bind to many types of pathogens, protecting the body against them by complement activation, opsonization for phagocytosis and neutralisation of their toxins.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Antigen: Full human IgG affinity purified from serum of four normal donors tested and found negative for Hepatitis B, Hepatitis C, HIV I and II antibodies using protein G. Purity > 95% by SDS-PAGE.
• Antibody Type: Secondary Antibody
• Concentration: 2 mg/ml
• Dilution range: WB:1:2,000-1:10,000, ELISA:20,000-100,000
• Form/Appearance: Polyclonal antibody supplied as a 500 µl (2 mg/ml) aliquot in PBS, 50% glycerol and 0.05% sodium azide.
• Conjugation: HRP
• Target: Immunoglobulin G
• Storage: For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
• Buffer/Preservatives: PBS,50% glycerol and 0.05% sodium azide
• Alternative names: Immunoglobulin G antibody.
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.