Anti-Hu TCR Vdelta2

• Catalog Number: orb1176619
• Category: Antibodies
• Description: TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.
• Clonality: Monoclonal
• Clone Number: B6
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: human recombinant TCR Vdelta2
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: TCR Vdelta2
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: TCRVD2
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human TCR Vdelta2 positive lymphocytes (red-filled) from TCR Vdelta2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).