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Catalog Number | orb43697 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD86. |
Clonality | Monoclonal |
Clone Number | BU63 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | B-lymphoblastoid cell line ARH 77 |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD86 |
Entrez | 942 |
UniProt ID | P42081 |
RRID | AB_10992842 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD86 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Rudolf-Oliveira, Renata Cristina Messores et al. Determination of normal expression patterns of CD86, CD210a, CD261, CD262, CD264, CD358, and CD361 in peripheral blood and bone marrow cells by flow cytometry Immunol Lett, 194, 44-55 (2018)
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD86 (BU63) PE antibody (concentration in sample 0.56 µg/ml).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD86 (BU63) PE antibody (concentration in sample 0.56 µg/ml).