CD64 Antibody

SKU: orb154423

Description

Mouse monoclonal antibody to CD64 which is a glycoprotein that is constitutively expressed on human monocytes and macrophages. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity and also acts in mediating humoral immune responses.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  Functional application: Blocking of IgG binding to the FcγR1. Flow cytometry: Recommended dilution: 1-4 μg/ml. Immunohistochemistry (paraffin sections and frozen sections): There can occur problems with paraformaldehyde fixation.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 10.1
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Target: CD64
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FcRI, IGFR1, FcgR1A

Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD64 (10.1) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD64 (10.1) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD64

UniProt

P12314

NCBI Gene ID

2209