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Anti-Hu CD5 Purified
Catalog Number: orb44290
| Catalog Number | orb44290 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD5. |
| Clonality | Monoclonal |
| Clone Number | MEM-32 |
| Tested applications | ELISA, FC, IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Crude thymus membrane fraction. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD5 |
| Entrez | 921 |
| UniProt ID | P06127 |
| RRID | AB_10994885 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-CD5 antibody, anti-T1 antibody Read more... |
| Note | For research use only |
| Application notes | ELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1. Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.
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