Anti-Hu CD5 Purified

• Catalog Number: orb44290
• Category: Antibodies
• Description: Mouse Monoclonal to CD5.
• Target: CD5
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by protein-A affinity chromatography.
• Immunogen: Crude thymus membrane fraction.
• UniProt ID: P06127
• RRID: AB_10994885
• Tested applications: ELISA, FC, IHC-P, IP, WB
• Application notes: ELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1. Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions.
• Antibody Type: Primary Antibody
• Clone Number: MEM-32
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CD5 antibody, anti-T1 antibody
• Note: For research use only
• Entrez: 921

Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.