Anti-Hu CD49a Purified

• Catalog Number: orb1088503
• Category: Antibodies
• Description: CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.
• Clonality: Monoclonal
• Clone Number: TS2/7
• Tested applications: FC, IHC-Fr, IP
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human CTL line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD49a
• Entrez: 3672
• UniProt ID: P56199
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-alpha 1 integrin antibody, anti-VLA-1 alpha a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of HeLa cells stained using anti-CD49a (TS2/7) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).