CD4 Antibody

SKU: orb44204

Description

Mouse monoclonal antibody to CD4

Research Area

Epigenetics, Immunology, Infectious Diseases, Stem Cells

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: peripheral blood, HPB T cell line. This antibody is excellent antibody for FC application. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood, HPB T cell line, non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: MEM-241
• Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc
• Target: CD4
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T4/Leu-3, L3T4

Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.

Separation of human CD4 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD4 (MEM-241) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-241) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD4

UniProt

P01730

NCBI Gene ID

920