CD4 Antibody

SKU: orb44034

Description

Mouse monoclonal antibody to CD4

Research Area

Epigenetics, Immunology, Infectious Diseases, Stem Cells

Images & Validation

• Tested Applications: FC, IP
• Reactivity: Human
• Application Notes:
  Immunoprecipitation: Excellent. Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: MEM-115
• Immunogen: Human thymocytes and T lymphocytes.
• Target: CD4
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T4/Leu-3, L3T4

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD4 (MEM-115) purified antibody, GAM-APC.

Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-human CD4 (MEM-241). Lane 1: original lysate of T cells; Lane 2: immunoprecipitate by negative control antibody; Lane 3: immunoprecipitate by anti-human CD4 (MEM-115).

Quick Database Links

Gene Symbol

CD4

UniProt

P01730

NCBI Gene ID

920