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| Catalog Number | orb222729 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD32 |
| Target | CD32 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified by protein-A affinity chromatography. |
| Immunogen | purified glycosylated recombinant human FcgammaRIIa2 |
| UniProt ID | P12318 |
| Tested applications | FC |
| Application notes | Flow cytometry: Recommended dilution: 2-6 μg/ml. |
| Antibody Type | Primary Antibody |
| Clone Number | 3D3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | FCG2, FCGR2A, IGFR2 |
| Research Area | Epigenetics, Immunology |
| Note | For research use only |
| Entrez | 2212 |

Mass cytometry (surface staining) of human peripheral blood with anti-CD32 (3D3) 167Er. Gated on singlets.

Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 µg/ml, GAM APC).
ELISA, FC, IF, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
Human | |
46.88-3000 pg/mL | |
16.58 pg/mL |
Human | |
15.63-1000 pg/mL | |
6.2 pg/mL |
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