Anti-Hu CD315 Purified

• Catalog Number: orb1678983
• Category: Antibodies
• Description: CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
• Target: CD315
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Immunogen: HeLa cells
• UniProt ID: Q9P2B2
• Tested applications: FC, IHC-Fr, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml
• Clone Number: 1F11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F
• Note: For research use only
• Entrez: 5738
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells stained using anti-human CD315 (1F11) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from A431 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).