Anti-Hu CD271 Purified

• Catalog Number: orb154382
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD271 which is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells.
• Clonality: Monoclonal
• Clone Number: NGFR5
• Tested applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Feline, Ferret, Human, Primate, Rabbit
• Isotype: Mouse IgG1
• Immunogen: Purified CD271 protein isolated from human melanoma cell line A875
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD271
• Entrez: 4804
• UniProt ID: P08138
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TNFRSF16 antibody, anti-NGFR antibody, anti-p
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.

Separation of SK-MEL-30 cells stained using anti-CD271 (NGFR5) purified antibody (concentration in sample 1.7 µg/ml, GAM APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5).