CD271 Antibody

SKU: orb154382

Description

Mouse monoclonal antibody to CD271 which is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells.

Research Area

Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Feline, Ferret, Human, Primate, Rabbit
• Application Notes:
  Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: NGFR5
• Immunogen: Purified CD271 protein isolated from human melanoma cell line A875
• Target: CD271
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNFRSF16, NGFR, p75NTR, p75NGFR

Mass cytometry (surface staining) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.

Separation of SK-MEL-30 cells stained using anti-CD271 (NGFR5) purified antibody (concentration in sample 1.7 µg/ml, GAM APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5).

Quick Database Links

Gene Symbol

CD271

UniProt

P08138

NCBI Gene ID

4804