You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Anti-Hu CD270 Biotin
Catalog Number: orb2657854
| Catalog Number | orb2657854 |
|---|---|
| Category | Antibodies |
| Description | CD270 is a type I transmembrane protein of the TNFR superfamily, which is expressed on resting T cells, monocytes, and immature dendritic cells. Its ligands, CD258 and CD272, differ in effect on CD270 signaling. Whereas binding to CD258 provides a costimulatory signal, binding to CD272 gives to the cell an inhibitory signal. CD270 also is recognized by herpes simplex glycoprotein D. CD258-CD270 interaction and signaling is implicated in macrophage-derived foam cell-mediated development of atherosclerotic lesions. |
| Clonality | Monoclonal |
| Clone Number | CW10 |
| Tested applications | ELISA, FC, ICC, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | recombinant human CD270 |
| Conjugation | Biotin |
| Target | CD270 |
| Entrez | 8764 |
| UniProt ID | Q92956 |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | TNFRSF14, TR2, ATAR, HVEA, HVEM, LIGHTR Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry. Anti-Hu CD270 Biotin antibody (concentration in sample 5 µg/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5 µg/ml, streptavidin APC, black-dashed histogram).
Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry. Anti-Hu CD270 Biotin antibody (concentration in sample 5 µg/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5 µg/ml, streptavidin APC, black-dashed histogram).
