CD270 Antibody (Biotin)

SKU: orb2657854

Description

CD270 Antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, FC, ICC, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: CW10
• Immunogen: recombinant human CD270
• Target: CD270
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

TNFRSF14, TR2, ATAR, HVEA, HVEM, LIGHTR

Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry. Anti-Hu CD270 Biotin antibody (concentration in sample 5 µg/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5 µg/ml, streptavidin APC, black-dashed histogram).

Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry. Anti-Hu CD270 Biotin antibody (concentration in sample 5 µg/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5 µg/ml, streptavidin APC, black-dashed histogram).

Quick Database Links

Gene Symbol

CD270

UniProt

Q92956

NCBI Gene ID

8764