Anti-Hu CD245 Purified

• Catalog Number: orb1742788
• Category: Antibodies
• Description: CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.
• Clonality: Monoclonal
• Clone Number: DY12
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: human NK-type YT2C2 cells
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD245
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: p220/240, DY12, DY35
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of monocytes (red-filled) stained using anti-human CD245 (DY12) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from monocytes cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD245 (DY12) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).