CD245 Antibody (PE)

• Catalog Number: orb1881232
• Category: Antibodies
• Description: CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.
• Target: CD245
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: human NK-type YT2C2 cells
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: DY12
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: p220/240, DY12, DY35
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD245 (DY12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD245 (DY12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).