CD1a Antibody (APC)

SKU: orb43817

Description

Mouse Monoclonal to CD1a.

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: HI149
• Immunogen: Human thymocytes
• Target: CD1a
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

T6, Leu-6, HTA1, FCB6

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml) and anti-human CD11c (BU15) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).

Separation of CD11c positive cells stained using CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml, red-filled) from CD11c positive cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.5 µg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml).

Quick Database Links

Gene Symbol

CD1a

UniProt

P06126

NCBI Gene ID

909