Anti-Hu CD173 Purified

• Catalog Number: orb1088490
• Category: Antibodies
• Description: CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
• Clonality: Monoclonal
• Clone Number: MEM-195
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: Human thrombocytes
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD173
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-Lewis Y antibody, anti-blood group H2 antigen
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD173 positive erythrocytes (red-filled) from human CD173 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).