Anti-Hu CD16 Purified

• Catalog Number: orb44123
• Category: Antibodies
• Description: Mouse Monoclonal to CD16.
• Clonality: Monoclonal
• Clone Number: MEM-168
• Tested applications: FC
• Reactivity: Human, Porcine, Primate
• Isotype: Mouse IgM
• Immunogen: Human granulocytes
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD16
• RRID: AB_10997790
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-CD16 antibody, anti-FACSgammaRIII antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. orb43568); Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168). Cells in the granulocyte gate were used for analysis.

Expression profiling on peripheral blood subsets using anti-human CD16 purified antibody (clone MEM-168). HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) with residual erythrocytes lysed with 10× diluted EXCELLYSE Live solution was added to the mixture of anti-human CD16 purified antibody (clone MEM-168, 2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, samples were centrifuged (670 g, 5 min.), supernatant removed and secondary antibody (GAM PE) was added to sample, vortexed and incubated for 20 min. Next, samples were washed twice (2 ml PBS, 670 g, 5 min.) and then optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. Next, samples are fixed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human CD16 purified antibody (clone MEM-168) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human CD16 purified antibody (clone MEM-168) was used in concentration 2 µg/ml in stained blood sample (2 x 10^6 cells).