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Catalog Number | orb222748 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CTLA4 |
Clonality | Monoclonal |
Clone Number | BNI3 |
Tested applications | FC, ICC, IHC-Fr, IP |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Human CD152-IgG heavy chain fusion protein |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD152 |
Entrez | 1493 |
UniProt ID | P16410 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti-ALPS5 antibody, anti-CD 152 antibody, anti-CD Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 2-5 μg/ml, Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.
Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.
FC, ICC, IHC-Fr, IP | |
Human | |
Monoclonal | |
Unconjugated |