CD152 Antibody

SKU: orb222748

Description

Mouse monoclonal antibody to CTLA4

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IP
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 2-5 μg/ml, Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: BNI3
• Immunogen: Human CD152-IgG heavy chain fusion protein
• Target: CD152
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTLA4, GSE, GRD4

Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD152

UniProt

P16410

NCBI Gene ID

1493