CD14 Antibody

SKU: orb44143

Description

Mouse Monoclonal to CD14.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FA, FC, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. orb43674). Functional application: The antibody MEM-18 completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain. Flow cytometry: recommended dilution: 4 μg/ml. Western blotting: Non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-18
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
• Target: CD14
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LPS-receptor, LPSR, MCLR

Quick Database Links

Gene Symbol

CD14

UniProt

P08571

NCBI Gene ID

929