CD123 Antibody (PE)

• Catalog Number: orb154527
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes CD123 that is also known as IL-3 receptor subunit alpha, IL-3R-alpha and IL3RA, is a single-pass type I membrane protein which is a member of the type I cytokine receptor family and Type 5 subfamily. CD123 is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. CD123 is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes.
• Target: CD123
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
• UniProt ID: P26951
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Antibody Type: Primary Antibody
• Clone Number: 6H6
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: IL3RA, MGC34174, hIL-3Ra
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 3563
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD123 positive Basophil granulocytes(red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).