CD11a Antibody

SKU: orb222798

Description

Mouse monoclonal antibody to CD11a

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FA, FC, IP
• Reactivity: Human
• Application Notes:
  Functional application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow cytometry: Recommended dilution: 1 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-83
• Immunogen: Human peripheral blood lymphocytes
• Target: CD11a
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LFA-1, LFA1A, ITGAL

Separation of human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD11a

UniProt

P20701

NCBI Gene ID

3683