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Item 1 of 4
Anti-Hu CD107b Purified Azide Free
Catalog Number: orb44718
| Catalog Number | orb44718 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD107b. |
| Clonality | Monoclonal |
| Clone Number | H4B4 |
| Tested applications | FC, ICC, IHC-Fr, IHC-P, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Human PBMC |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-2 μg/ml. Immunohistochemistry: Recommended dilution: 2-8 μg/ml. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD107b |
| Entrez | 3920 |
| UniProt ID | P13473 |
| RRID | AB_10992271 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Alternative names | Anti-CD107b antibody, anti-LAMP-2 antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-2 μg/ml. Immunohistochemistry: Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor surface staining of human anti-IgE antibody stimulated mononuclear cells stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) and anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD107b positive CD203c positive basophil granulocytes (red-filled) from CD107b negative CD203c positive basophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).
Anti-Hu CD107b Purified (clone H4B4) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of SK-MEL30, MCF-7, THP-1, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4B4 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD107b molecules were detected at ~95-125 kDa in all tested cell lines under both reducing and non-reducing conditions.
