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Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1:1000, IHC-P: 1:100-1:300, IF: 1:100-1:500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within human HSP60. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
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Immunohistochemical analysis of HSP60 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
HSP60 Antibody (orb705387)
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