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Catalog Number | orb2609077 |
---|---|
Category | Antibodies |
Description | Anti-Hsp27/HSPB1 Antibody (monoclonal, 3H3). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3H3 |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | E.coli-derived human Hsp27/HSPB1 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27. |
Antibody Type | Primary Antibody |
Form/Appearance | Lyophilized |
Conjugation | HRP |
MW | 27 kDa |
UniProt ID | P04792 |
Storage | Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Heat shock protein beta-1; HspB1; 28 kDa heat shoc Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500μg/ml |
Expiration Date | 12 months from date of receipt. |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
iFluor647 |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
PE |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
APC |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
FITC |